Best Practices for Accurate E. coli Water Results

To get accurate water test results, samples must be handled with care. Though different water testing labs may have specific standard operating procedures, there are universal water sample collecting practices that must be adhered to. They include; the type of water sampling bottles to be used, holding duration and preservation techniques. E. coli water testing bottles must be sterilized and sizeable to hold enough water sample.

Features of an Ideal E. coli Water Testing Bottle by Eco-sensa

The water sampling bottle is designed to facilitate E. coli and total coliforms water portability testing. As such, it is a 120 bottle made of polyethylene. It features a 100 ml fill line leaving a 20 ml airspace that is required during lab tests and also prevents the sample from spilling during transportation. The sampling bottles come with sodium thiosulfate certified to neutralize 10mg/L of chlorine in the water.

The bottle features a leak-proof seal that withstands shaking to prevent leakages during sample transportation. Moreover, sampling bottles come with a removable tamper proof seal; do not use a bottle to collect E. coli water testing sample if the seal has been broken. The bottle is sterile to ensure accurate test results.Untitled

How to Collect E. coli Water Testing Sample

A proper sample collection procedure must be followed to obtain accurate results;

– Start by obtaining a sterile sampling bottle and ensure that the tamper-proof seal is intact. Label the bottle, indicate the sample collection date, and location. Wash your hands and wear disposable and clean gloves.

– Turn on a cold water tap and allow the water to flow for about 5 minutes to clear the line and ensure that the water is cold. Do not rinse or clean the sampling bottle. Place the bottle cap facing up to avoid contamination. The bottle and cap should not come into contact with the collected water sample and air.

– Reduce the tap water flow to ensure that it flows smoothly and to prevent splashing. Fill the bottle to the indicate fill line. If the sampling bottle does not have a fill line, leave substantial air space.

– Cap the sample bottle immediately after collecting the water and ensure that the cap is tight enough to avoid leakages. Note that, you should not touch the inside of the cap or bottle with your hands.

– Submit the E. coli water test sample to a licensed laboratory within 24 hours to avoid unpredictable changes in concentration of samples and bacterial composition. If you will not send the sample immediately, keep it refrigerated at 4 to 10 degree Celsius. The sample should not freeze.

– Water sampling bottles should be shipped in coolers or foam pack containers with ice packs and ice bags. The e. coli testing water bottle must be kept at 4 to 10 degrees Celsius during transportation. Loose ice can contaminate the samples.


The Eco-sensa sampling bottles are sterile and meet set E. coli water testing bottle requirements to ensure that collected samples are representative of the bacteria being tested.